| Facility | Hogwarts |
| Analyst | John Doe |
| Analysis started | 2025-04-03 05:23:58 |
| Analysis completed | 2025-04-03 05:23:58 |
| Wall time | 0:0:0 hours |
| locus | COI |
| preliminary_id | Phoridae |
| taxa_of_interest |
Megaselia scalaris |
| country | India |
| host | Fish meal |
| sample_id | VE25-1434_COI |
| Query DNA sequence |
Inconclusive
The analyst should attempt subjective species identification at the genus level.
Reasoning - Flag 1D:
At least one candidate species matched with moderate stringency (identity ≥93.5% and <98.5%).
| Preliminary morphology ID confirmed | NA |
|
Inconclusive taxonomic identity (Flag 1D) |
|
| Taxa of interest ruled out | False |
|
Flag 2A: Taxon of interest NOT detected Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries) Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus |
|
Flag 1D:
The analyst should attempt subjective species identification at the genus level
At least one candidate species matched with moderate stringency (identity ≥93.5% and <98.5%)
Candidate hits must meet ONE of these criteria:
| Minimum alignment length |
400bp
|
| Minimum query coverage |
85.0%
|
Candidate hits are then classified as follows:
| Classification | Alignment identity | Number of hits | Number of species |
|---|---|---|---|
| STRONG MATCH | ≥ 98.5% | 0 | 0 |
| MODERATE MATCH | ≥ 93.5% | 7 | 1 |
| NO MATCH | < 93.5% |
Hits per candidate species (top 10 candidates only)
| Species | Hits | Identity | E-value |
|---|---|---|---|
| Dohrniphora cornuta | 7 | 97.89999999999999% | 0.0 |
This sections shows the taxa of interest specified by the submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.
| Taxon of interest | Match rank | Match taxon | Match species | Match accession | Match identity |
|---|---|---|---|---|---|
| Megaselia scalaris | - | - | - | - | - |
See the Database coverage section to see database coverage for taxa of interest.
This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.
Independent sources
Flag 4A:
Reference sequences are from diverse sources and therefore likely to be reliable
Reasoning: Matching sequence records for this species are from >5 independent sources
(found 6 sources)
6 Independent Sources
The matching reference sequences for this species have been annotated by 6 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG947378 | False |
Moskowitz,N.A. Roland,A.B. Fischer,E.K. Ranaivorazo,N. Aguilar,M.T. Caldera,S.M. Chea,J. Cristus,M. Crowds,J.P. Demessie,B. Desjardins-Park,C.R. Effenberger,A.H. Flores,F. Giles,M. He,E.Y. Izmaylov,N.S. Lee,C. Nadeem,B. Pagel,N.A. Phu,K. Rosen,L.U. Seda,D.A. Shen,Y. Vargas,S. Weiss,H.S. Murray,A.W. Abebe,E. Vidoudez,C. Trauger,S.A. Vences,M. O'Connell,L.A. |
Seasonal changes in diet influences toxicity in the Climbing Mantella frog (Mantella laevigata) | Unpublished |
| MG947378 | False |
Moskowitz,N. Abebe,E. Desjardins-Park,C. He,E. Rosen,L. Seda,D. Caldera,S. Aguilar,M. Lee,C. Izmaylov,N. Phu,K. Weiss,H. |
Direct Submission | Submitted (13-FEB-2018) Biology, Stanford University, 371 Serra Mall, Stanford, CA 94305, USA |
Source 2
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| HQ984503 | False | iBOL Data Release | Unpublished | |
| HQ984503 | False | Direct Submission | Submitted (24-JAN-2011) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Rd West, Guelph, Ontario N1G2W1, Canada |
Source 3
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| KR666968 | False |
Hebert,P.D. Ratnasingham,S. Zakharov,E.V. Telfer,A.C. Levesque-Beaudin,V. Milton,M.A. Pedersen,S. Jannetta,P. deWaard,J.R. |
Counting animal species with DNA barcodes: Canadian insects | Philos. Trans. R. Soc. Lond., B, Biol. Sci. 371 (1702) (2016) In press |
| KR666968 | False |
Hebert,P.D.N. Ratnasingham,S. Zakharov,E.V. Dewaard,J.R. |
Direct Submission | Submitted (11-MAY-2015) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road EAST, Guelph, Ontario N1G2W1, Canada |
| KR659695 | False |
Hebert,P.D. Ratnasingham,S. Zakharov,E.V. Telfer,A.C. Levesque-Beaudin,V. Milton,M.A. Pedersen,S. Jannetta,P. deWaard,J.R. |
Counting animal species with DNA barcodes: Canadian insects | Philos. Trans. R. Soc. Lond., B, Biol. Sci. 371 (1702) (2016) In press |
| KR659695 | False |
Hebert,P.D.N. Ratnasingham,S. Zakharov,E.V. Dewaard,J.R. |
Direct Submission | Submitted (11-MAY-2015) Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road EAST, Guelph, Ontario N1G2W1, Canada |
Source 4
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG293276 | False | Dewaard,J.R. | BIOUG Archive GGBN Data Release | Unpublished |
| MG293276 | False | Dewaard,J.R. | Direct Submission | Submitted (30-OCT-2017) Collections, Centre for Biodiversity Genomics, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada |
Source 5
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| JQ941749 | False |
Wang,J.-F. Sheng,X.-X. Liu,G.-C. |
DNA-based identification on sexual dimorphism of Phoridae (Diptera) from China | Unpublished |
| JQ941749 | False |
Wang,J.-F. Sheng,X.-X. Liu,G.-C. |
Direct Submission | Submitted (16-APR-2012) Shenyang University, Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, 21 Wanghua South Street, Dadong District, Shenyang, Liaoning 110044, P.R. China |
Source 6
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MN832849 | False |
Feng,D. Li,J. |
The complete mitochondrial genome of two scuttle flies, Megaselia spiracularis Schmitz and Dohrniphora cornuta (Bigot) (Diptera: Phoridae) | Unpublished |
| MN832849 | False |
Feng,D. Li,J. |
Direct Submission | Submitted (14-DEC-2019) College of Life Science and Engineering, Shenyang University, Shenyang University, No. 21, Wanghua South Street, Dadong District, Shenyang, Liaoning 110044, China |
The target taxa include candidate species, the preliminary morphology ID, and any taxa of interest provided by the submitter. Each of these taxa are independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of the target taxon. Insufficient coverage of a taxon can result in that taxon not be correctly identified as the taxonomic identity of the sample. For example, if the sample is Homo sapiens, but Homo sapiens sequences are not included in the reference database, the analysis will be unable to identity Homo sapiens as the correct taxonomic identity, and will most likely assign the closest relative with reference data as the taxonomic identity.
Database coverage
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
Flag 5.1A:
The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)
There are 15 sequences in the reference database for Dohrniphora cornuta at the given locus COI.
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Flag 5.3C:
Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: No species in genus have been observed in the country of origin
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Database coverage
Flag 5.1NA:
This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species
Flag 5.1NA:
This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species
There are NA sequences in the reference database for Phoridae at the given locus COI.
Database coverage
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
Flag 5.1A:
The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)
There are 48 sequences in the reference database for Megaselia scalaris at the given locus COI.
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Flag 5.3A:
Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.
The following resources can be used to ensure that the given taxonomy is legitimate and current.
| Taxa | Database |
|---|---|
| General | GBIF |
| General | ITIS |
| Mealybugs & scale | ScaleNet database |
| Thrips | Thripswiki |
| Spider Mites | Spider Mites Database |
| Psocodea (Barklice, Booklice, and Parasitic Lice) | Psocodea Species File Online |
| Orthoptera | Orthoptera Species File Online |
| Drosophilidae | TaxoDros |
| Diptera |
Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum |
| Aphids | Aphid Species File |
| Ants |
AntWeb
AntCat |
| Lepidoptera (butterflies and moths) | The Global Lepidoptera Names Index |
| Gracillariidae (primitive moths) | Global Taxonomic Database of Gracillariidae |
| Pyralidae (pyralid moths) | Global Information System on Pyraloidea |
| Tortricidae (tortrix moths) | Tortricidae Resources on the Net |